Zavedení
This manual provides essential information for the proper use, setup, and maintenance of your Labnet International E0162 Small Gel Tray Set. This set is designed for casting small agarose or polyacrylamide gels, commonly used in molecular biology laboratories for electrophoresis applications. Please read this manual thoroughly before initial use to ensure optimal performance and longevity of the product.
Obsah balení
The image displays the complete Labnet electrophoresis system, which includes the E0162 Small Gel Tray Set. The set itself typically contains the following components:
- Small Gel Tray (12.5 cm W x 6 cm L): A clear, rectangular tray designed to hold the gel during casting and electrophoresis. The product description indicates a pack of two trays.
- Gel Combs: Multiple comb units with various well configurations (e.g., 10-well, 15-well) for forming sample wells in the gel. These are typically white plastic.
- Casting Stand: A white plastic base with slots to securely hold the gel tray and combs during the gel casting process.
Please verify that all listed components are present and undamaged upon unpacking.
Nastavení
- Příprava: Ensure all components of the gel tray set are clean and dry before use.
- Assemble Casting Stand: Place the casting stand on a level surface.
- Insert Gel Tray: Carefully slide the small gel tray into the designated slot on the casting stand. Ensure it is seated firmly and level.
- Position Combs: Select the appropriate gel comb for your experiment. Insert the comb into the slots on the casting stand, ensuring the teeth are suspended above the bottom of the gel tray, creating a gap for the gel solution. The depth of the comb insertion determines the well depth.
- Seal Tray (if necessary): For some casting systems, the ends of the gel tray may need to be sealed with tape or rubber gaskets to prevent leakage of the molten gel solution. Refer to your specific electrophoresis system's manual for details on sealing.
Operating Instructions (Gel Casting)
This section outlines the general procedure for casting a gel using the Labnet Small Gel Tray Set. Specific gel preparation protocols (e.g., agarose concentration, buffer type) should be followed according to your experimental requirements.
- Příprava gelového roztoku: Prepare your desired agarose or polyacrylamide gel solution according to standard laboratory protocols. Ensure the solution is homogeneous and at the appropriate temperature for pouring.
- Nalijte gel: Carefully pour the molten gel solution into the assembled gel tray on the casting stand. Pour slowly to avoid air bubbles. Ensure the gel solution covers the bottom of the comb teeth.
- Polymerization: Allow the gel to polymerize completely. This typically takes 20-60 minutes for agarose gels at room temperature, depending on the concentration. For polyacrylamide gels, polymerization time will vary based on catalyst concentration.
- Odstraňte hřeben: Once the gel has fully polymerized and is firm, carefully remove the gel comb by pulling it straight up. Avoid twisting or wiggling the comb, as this can damage the wells.
- Remove Gel Tray: Gently remove the gel tray with the cast gel from the casting stand. The gel is now ready for transfer to an electrophoresis tank.
Údržba a péče
Proper maintenance ensures the longevity and performance of your gel tray set.
- Čištění: After each use, thoroughly rinse the gel tray, combs, and casting stand with distilled water to remove any residual gel or buffer. For stubborn residues, a mild laboratory detergent can be used, followed by extensive rinsing.
- Sušení: Allow all components to air dry completely or wipe them with a lint-free cloth before storage.
- Skladování: Store the gel tray set in a clean, dry place, away from direct sunlight and extreme temperatures. Avoid stacking heavy objects on top of the trays or combs to prevent warping or damage.
- Inspekce: Periodically inspect the gel tray and combs for any cracks, scratches, or deformities that could affect gel casting or electrophoresis results. Replace damaged components as needed.
Odstraňování problémů
| Problém | Možná příčina | Řešení |
|---|---|---|
| Gel leakage during casting | Improper sealing of the tray ends; tray not seated correctly in casting stand; damaged tray. | Ensure proper sealing with tape or gaskets (if applicable). Verify tray is firmly seated. Inspect tray for cracks and replace if damaged. |
| Uneven gel polymerization | Gel solution not homogeneous; temperature fluctuations during polymerization; casting surface not level. | Ensure gel solution is thoroughly mixed. Cast gel in a stable temperature environment. Use a level surface for casting. |
| Damaged or distorted wells after comb removal | Comb removed too early (gel not fully polymerized); comb removed at an angle or with force. | Allow gel to fully polymerize before removing comb. Remove comb slowly and straight up. |
Specifikace
- Název produktu: Labnet International E0162 Small Gel Tray Set
- Číslo modelu: 1196M14PK
- Gel Tray Dimensions: 12.5 cm (Width) x 6 cm (Length)
- Množství: Pack of 2 Gel Trays
- Materiál: High-quality clear acrylic/plastic (typical for gel trays)
- Výrobce: Labnet International, Inc.
Záruka a podpora
For warranty information, technical support, or to inquire about replacement parts for your Labnet International E0162 Small Gel Tray Set, please contact Labnet International, Inc. directly or refer to their official webUschovejte si účtenku jako doklad o koupi pro případné reklamace.
Kontaktní údaje: Please refer to the official Labnet International webNejnovější kontaktní údaje naleznete na webu nebo na obalu produktu.
