Návod k obsluze spektrofotometru ACCURIS INSTRUMENTS NS1000 SmartDrop Nano

Počáteční kontrola
When you first receive the SmartDropTM L Nano Spectrophotometer,carefully inspect the instrument and all included accessories for any damage or missing items. If there are any issues, contact Benchmark Scientific or your local distributor immediately.

Bezpečnostní upozornění a pokyny

Je důležité dodržovat tyto bezpečnostní pokyny:

Read the user manual carefully before operating the instrument.
Operate the instrument as per the guidelines to prevent injury or damage.
Only skilled laboratory personnel with proper training should operate the device.

Avoid attempting to repair the instrument without guidance from Accuris Instruments.
Always power off the instrument after use and unplug it. Cover the instrument to prevent dust accumulation.

Clean the pedestal and housing regularly using appropriate materials.

Údržba

Pro optimální výkon je nezbytná pravidelná údržba:

Clean the pedestal with a soft cloth dampened with deionized water.
Clean the instrument housing with a soft cloth dampzalité malým množstvím alkoholu.

Tento produkt je určen pouze pro výzkumné účely

Děkuji za nákupasing the SmartDrop™ L Nano Spectrophotometer. This user manual details the instrument’s features, specifications, as well as complete operating instructions; please read it carefully before operation. Keep this user manual for later use. The SmartDrop™ L is designed for research use only.

Důležité:
Uschovejte si krabici a obalový materiál tohoto nástroje. Pokud je vyžadován servis, budete potřebovat krabici k odeslání přístroje našemu servisnímu oddělení.

Počáteční kontrola

Při prvním otevření obalu prosím zkontrolujte přístroj a veškeré přiložené příslušenství. Pokud zjistíte, že je cokoli poškozené nebo chybí, okamžitě kontaktujte společnost Benchmark Scientific nebo místního distributora.

BENCHMARK SCIENTIFIC / ACCURIS INSTRUMENTS
PO Box 709
Edison, NJ 08818

USA

Telefon: 908-769-5555

Webmísto: www.benchmarkscientific.com / www.accuris-usa.com

Bezpečnostní upozornění a pokyny

Důležité informace pro bezpečné použití
Uživatelé by měli rozumět tomu, jak tento nástroj používat, než jej začnou používat. Před uvedením do provozu si prosím pečlivě přečtěte tento návod.

Jakákoli nesprávná obsluha může způsobit zranění. Přečtěte si prosím pozorně tento návod a pracujte bezpečně podle pokynů.

Provoz a údržba
Provoz a údržba přístroje by měly být v souladu se základními pokyny a varováními níže. Nesprávná obsluha nebo údržba budou mít škodlivé účinky na životnost, výkon a bezpečnostní funkce přístroje.

The instrument is a normal indoor instrument which conforms to classⅠof the GB 4793.1 standard.

Tento přístroj je určen pro použití v laboratorním prostředí. Zařízení musí obsluhovat zkušený laboratorní personál s odpovídajícím školením.
Aby se předešlo zranění nebo ztrátě záruky, operátor by se neměl pokoušet opravit přístroj bez výslovného pokynu společnosti Accuris Instruments. Je-li vyžadován servis, kontaktujte společnost Accuris Instruments nebo místního distributora za účelem opravy.

Před zapnutím se ujistěte, že voltagPoužitý splňuje elektrické požadavky přístroje uvedené na typovém štítku. Pokud je elektrický kabel poškozen, vyměňte jej za stejný typ kabelu. Před vytažením zástrčky ze zásuvky pevně držte zásuvku. Netahejte za elektrický kabel.
Přístroj by měl být instalován v prostředí se standardní pokojovou teplotou, nízkou prašností, nízkou vlhkostí a mimo přímé sluneční světlo, elektromagnetické rušení a zdroje tepla. Neblokujte ventilační otvory na přístroji.

Po skončení používání vždy přístroj vypněte. Odpojte napájecí kabel a přikryjte přístroj látkou nebo plastovou fólií, aby se do krytu nedostalo nadměrné množství prachu.
Okamžitě vytáhněte zástrčku konektoru z elektrické zásuvky a kontaktujte prodejce v případě:
- Kapalina vniklá do krytu.
- Abnormální provoz: jako je abnormální zvuk nebo zápach.
- Přístroj spadl nebo došlo k poškození krytu.
- Jakákoli porucha.

Údržba
The pedestal should be cleaned regularly using a soft cloth dampened with deionized water. The instrument housing should be cleaned regularly using a soft cloth dampzalité malým množstvím alkoholu.

Zavedení

The SmartDrop™ L nano spectrophotometer measures 1.0µL – 2.0µL samples with high accuracy and reproducibility and employs surface tension to position the sample for measurement. The system includes a cuvette slot for OD600 readings & a built-in printer for printing results. The SmartDrop L can measure highly concentrated samples without dilution (100X concentration of samples measured by a standard cuvette spectrophotometer).

Klíčové vlastnosti

User-Friendly Input & Operation – Touch screen for programming and operation (a mouse can also be connected).

Multifunctional software for Nucleic Acids, Protein A280, & OD600 measurements.
dsDNA detection range from 10ng/µL – 2500ng/µL.
Fast & accurate measurements (< 6 seconds).
2 cuvettes included (optical glass) for OD600 measurements.

Specifikace

Required Installation Environment Environmental Temperature: 5°C～35°C

Relativní vlhkost: ≤ 70 %
Vstupní objemtage: DC 24V, 2A ( Adapter CSA, UL, CE marked)

Specifikace

Model		SmartDrop™ L (NS1000)
Minimálně SampVelikost le		1.0μL – 2.0μL
Délka cesty		0.5 mm
Light Source / Life		UV LED / 8000h
Typ detektoru		UV Silicon Photocell
Rozsah vlnových délek		260, 280, 600nm
Přesnost vlnové délky		± 1 nm
Absorbance Precision		0.005Abs
Conc. Accuracy		±5ng [10,500) ±2% [500,2000) ±3 % [2000,2500]
Absorbance Range		0.2-50 (10mm equivalent)
Detekce Rozsah koncentrace		10ng/μL dsDNA ～ 2,500ng/μL dsDNA
Čas detekce		< 6 sekundy
OD600	Abs range	0~4.000 Abs
	Abs stability	[0,3）≤0.5% [3,4）≤2%
	Abs opakovatelnost	[0,3）≤0.5%, [3,4）≤2%
	Abs Precision	[0,2）≤0.005A, [2,3）≤1%， [3,4）≤2%
svtage vstup		DC 24V, 2A
Moc		25W
Rozměry()Š × h × v）		20.8cm × 28.0cm ×18.6cm / 8.2in x 11.0in x 7.3in
Hmotnost		3.6 kg / 7.9 liber

Nástroj skončilview

Struktura

Přední

Sample Size Requirements
Surface tension is a critical factor in the formation of the sample column for measurement. The hydrophobic interactions between water molecules in a sample solution is key in creating & maintaining surface tension. The presence of solutes (proteins, DNA, RNA, salt ions, detergent molecules) significantly reduces surface tension and hinders the formation of the sample column. For most samples, a 1μL sample size is enough; however, to ensure accurate and precise measurements, a 2μL sample size is recommended to allow the formation of the sample column for measurement.

To ensure precise and accurate measurements, it is essential that a complete liquid column forms between the upper pedestal and lower pedestal. It is recommended that a precision pipettor (0-2μL) be used to dispense samples.

Dispensing Samples onto The Lower Pedestal
Lift the upper pedestal and pipette the sample (1.0µL – 2.0μL) onto the lower pedestal (Fig. 3).

Lower the upper pedestal onto the sample to form the sample column (Fig. 5).

Poznámka: Please exercise caution when lowering the upper pedestal onto the sample.

Aby se zabránilo sample carryover, use a soft laboratory wipe and deionized water to clean both pedestals in between sample measurements (Fig. 6).

OD600 Measurement
The SmartDrop L includes a cuvette slot for OD600 measurements. Lift the upper pedestal to expose the cuvette slot. Select the OD600 interface on the touch screen.

Insert and read a “blank” as required for the experiment. Then add 2~3mL of sample into the cuvette. Place the cuvette into the slot and start the measurement (Fig. 7).

Poznámka: The direction of the light path is shown by the red arrow in the figure above. Please ensure the cuvette is loaded with the correct orientation.

Programování a provoz

Spouštěcí rozhraní
Upon powering on the instrument, it will perform a self-check, and the start-up screen will be displayed (Fig. 8)

Rozhraní hlavní nabídky

After start-up, the main menu interface will be displayed. There are 4 options: Nucleic Acids, Protein A280, OD600, & System Settings

Nucleic Acids Interface
Beer-Lambert’s Law for DNA/RNA quantitation

The following “Beer－Lambert” equation is used to calculate the concentration of nucleic acids:

C＝Sample DNA concentration, unit : ng/μL
A＝Sample absorbance, unit : A
ε＝extinction coefficient, unit: ng-cm/μL
b＝Path Length, unit: cm

Standard DNA/RNA extinction coefficients :

dsDNA：50ng-cm/μL
ssDNA：33ng-cm/μL
RNA：40ng-cm/μL

Když sample column is used, highly concentrated nucleic acid samples can be measured without dilution using a 0.5mm path length. The SmartDrop L will measure and display sample absorbance values of the 10mm pathlength equivalent of up to 50 A.
The SmartDrop L will accurately measure dsDNA samples up to 2500ng/μL without dilution.

Select “Nucleic acid” from the main menu to enter the Nucleic Acid Interface:

Fig 10; there are three tabs in the Nucleic Acids Interface: Nucleic Acids, Report, and Help.

: Sample ID name has a default value of the current date and time. Users can rename the sample ID. One sample ID can contain up to 1000 measurement values.
: Vyberte sample type: DNA-50 for dsDNA, RNA-40 for RNA, ssDNA-33 for ssDNA. For a different nucleic acid type, select “others” and enter the extinction coefficient.
: Perform a blank reading. This step is essential before measurement. Blank absorbance values are typically in the range of 0.004-0.03 Abs and are valid for up to 30 minutes. The instrument will automatically remind the user to perform another blank reading after 30 minutes.
: Spectrum normalization; The baseline is automatically set to the absorbance of the sample at 365nm and can be modified. This feature can remove spectroscopic signals from sample measurements by subtracting the measured absorbance at a specified baseline correction wavelength from the absorbance values at all wavelengths of a measured sample.
- Poznámka: If baseline calibration is not performed, the spectroscopic signals will not be separated from interference/background effects and will lead to inaccurate results.
The icon appears in the upper right corner to indicate an error in reading blank/sample volumes. Please clean and wipe the pedestal and perform another blank reading. If the problem persists, contact Accuris Instruments.

Operace:

Nastavte Sample ID.
Clean the upper and lower pedestals with a lint-free wipe, add 2μL buffer solution to perform a blank reading.
Clean the buffer solution on the pedestals with a wipe.
Measure a 2μL sample volume and click “Measure” to detect the sample. Note: A blank reading must be performed prior to sample measurements. The sample volume must be equivalent to the volume used to set the blank reading.

Clean and dry the pedestal between measurements.

Sample concentration and absorbance ratios will display on the left side of the interface (Fig. 12).

Conc.：Calculated nucleic acid concentration.
A260: Sample absorbance at 260nm (10mm pathlength equivalent).
A280: Sample absorbance at 280nm (10mm pathlength equivalent).
A260/A280: The ratio of corrected absorbance values at 260nm to corrected absorbance values at 280nm. An A260/A280 ratio of ~1.8 is generally accepted as pure for DNA (~2.0 for RNA). Acidic solutions will under-represent the A260/A280 ratio by 0.2 – 0.3 units, whereas basic solutions will over-represent the A260/280 ratio by 0.2 – 0.3 units.

Nucleic Acid Report Interface

Select the “Report” tab at the top of the Nucleic Acid Interface (Fig. 13). Users can select previously saved results by the file jméno.

Protein A280 Interface

Zavedení
The Protein A280 interface can be used to quantify purified proteins that contain amino acids such as tryptophan, tyrosine, or cys-cys disulfide bonds. These amino acids exhibit peak absorbance at 280nm. The following sample types can be selected:
“A280”, “BSA”, “IgG”, “Lysozyme”, & “Others”.

This interface does not require the generation of a standard curve. Sample absorbance values (260nm & 280nm) and A260/A280 ratios can be measured and displayed. A baseline correction can be used for normalization. Like the Nucleic Acids interface, the Protein A280 interface automatically displays 10mm pathlength equivalent data.

Protein A280 Interface
Select “Protein A280” from the main menu interface.

Obr. 14; There are three options at the top of the screen, Protein A280, Report, and Help.

: Sample ID name has a default value of the current date and time. Users can rename the sample ID. One sample ID can contain up to 1000 measurement values.
: Vyberte sample type: A280, BSA, IgG, Lysozyme. Select “others” and type in the extinction coefficient.

: Perform a blank reading. This step is essential before measurement. Blank absorbance values are typically in the range of 0.004-0.03 Abs and are valid for up to 30 minutes. The instrument will automatically remind the user to perform another blank reading.
The icon appears in the upper right corner to indicate an error in reading blank/sample volumes. Please clean and wipe the pedestal and perform another blank reading. If the problem persists, contact Accuris Instruments.

Operace:

Nastavte Sample ID.
Clean the upper and lower pedestals with a wipe, add 2μL buffer solution to perform a blank reading.

Clean the buffer solution on the pedestals with a wipe.
Measure a 2μL sample volume and click “Measure” to detect the sample. Note: A blank reading must be performed prior to sample measurements. The sample volume must be equivalent to the volume used to set the blank reading.
Clean and dry the pedestal between measurements.

Sample concentration and absorbance ratios will display on the left side of the interface (Fig. 16).

Conc.: Calculated protein concentration.
A260: Sample absorbance under 260nm (10mm pathlength equivalent).
A280: Sample absorbance under 280nm (10mm pathlength equivalent).
A260/A280: The ratio of corrected absorbance values at 260nm to the corrected absorbance values at 280nm.This ratio can be used as a secondary measure of nucleic acid purity. An A260/A280 ratio within the range of ~1.8 – 2.2 as pure for nucleic acids. Lower ratio values indicate the presence of contaminants that absorb strongly at or near 280nm.

Protein A280 Report Interface

Note: This interface is similar to the Nucleic Acids detection interface (see section 3).

OD600 Interface

Zavedení
The OD600 function allows for measuring absorbance values at 600nm. It is commonly used for measuring the growth rate of bacterial cell cultures by measuring absorbance of the culture in growth media at 600nm. The Beer-Lamber equation is used to calculate the concentration (see section 3). This interface does not require the generation of a standard curve.

OD600 Measurement

Operace

In the main instrument interface, click in OD600 icon.
Nastavte Sample ID.
Lift the spectrophotometer arm and place a 1.5 mL cuvette containing blanking solution (e.g. media) into the cuvette port.
On the instrument screen, select ”blank”.
Replace the cuvette containing blank solution with the cuvette containing the sample in the cuvette port and select “Sample” on the screen of the instrument.
Repeat the previous step until all samples have been read by the instrument.

OD600 Report Interface

Poznámka: This interface is similar to the Nucleic Acids detection interface (see section 3).

Rozhraní nastavení systému
Click “System” on the main interface to enter the System Settings Interface (Fig. 20).

Date & Time Settings Interface
Click the “Time” icon to enter the date and time settings interface (Fig. 21).

Click “Set date” to enter the Date Settings Interface (Fig. 22). Click “Set Time” to enter the Time Settings Interface (Fig. 23).

Vytisknout
Click the “Print” icon to set the print mode.

Jas
Click the “Brightness” icon to enter the Brightness Settings Interface. Use the slider to adjust the brightness of the display.

Odstraňování problémů

Chyba	Analýza	Odstraňování problémů
Instrument does not power on.	No power supply, Switch defective, Power adapter defective.	Zkontrolujte napájení, Replace the switch, Contact Accuris Instruments.
Measurement results not precise	Sample column unformed,	Přidat sample again, make sure the liquid column formed well, Clean the pedestals,
	Pedestal contaminated	Contact Accuris Instruments.
OD600 module failure	Poor connection between cable and board.	Contact Accuris Instruments.
Insufficient light intensity error	Analysis module defective, optical fiber broken.	Contact Accuris Instruments.
Touch screen error	Power supply does not have effective grounding.	Provide effective grounding power supply.
Časový limit komunikace	Analysis module communication failure.	Contact Accuris Instruments.

Nejčastější dotazy

Can I use the SmartDropTM L Nano Spectrophotometer for purposes other than research?

No, this product is designed for research use only. Any other use may result in improper functioning or damage.

What should I do if I find the instrument damaged upon initial inspection?

Contact Benchmark Scientific or your local distributor immediately to address any issues with the instrument.

Dokumenty / zdroje

ACCURIS INSTRUMENTS NS1000 SmartDrop Nano Spectrophotometer [pdfNávod k obsluze
NS1000, NS1000 SmartDrop Nano Spectrophotometer, SmartDrop Nano Spectrophotometer, Nano Spectrophotometer, Spectrophotometer

Reference

Uživatelská příručka

ACCURIS INSTRUMENTS NS1000 SmartDrop Nano Spectrophotometer

Návod k použití produktu